Biochemistry - Protein purification

Biochemistry - Protein purification

​The Protein Purification Service Group offers support to the Institute's researchers in the following areas:

  • Analytical and semi-preparative analysis of various biological molecules with UPLC/ HPLC/ FPLC using UV/mass and fluorescence spectroscopy
  • All chromatographic methods are used (ion exchange, IMAC, gel filtration, affinity, HIC, reversed phase)
  • Development and optimization of purification strategies for proteins of different origin (prokaryotic, eukaryotic, fusion and membrane proteins etc.) from cultivation to analysis
  • protein biochemical methods (gel electrophoresis, immunoprecipitation, labeling of proteins, peptides and lipids, refolding of denatured proteins, enzymatic assays)
  • Generation of monoclonal antibodies

The instrumental equipment includes (selection):​

  • UPLC system H-Class with MassSpec of the company Waters
  • HPLC system Aquity-ARC of the company Waters
  • ÄKTA-Purifier-FPLC system of the company GE
  • ​Biacore X-100 from GE​​
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